by Dr. Betty Martini, D.Hum
If you wonder why so many
children have ADD, it's because aspartame destroys the
brain - especially in the area of learning.
This is a study from
Norway in 2001.
Dr. Betty Martini,
Mission Possible International
9270 River Club Parkway
Duluth, Georgia 30097
Aspartame toxicity Center,
Introduction: Aspartame (ASM) is a product that
was originally made for diabetics, but today ASM is widely used by
healthy people as artificial sweetener in many food products.
The main goal with this research was to
see whether ASM was harmful to brain cells (cerebellar
granule cells). We wanted to check if the damage to the neurons
are connected to the N-methyl-D-aspartate (NMDA)-receptors on these
Procedure: Brain cells from 7 day
old mice were used. They were cultured in 24 well dishes and
different quantities of ASM were added. After 7 days the cultures
were analysed by two different tests:
Lactate dehydrogenases (LDH)
test, which gives a picture of cell death (LDH leakage to
the medium in which the cells were cultured).
(MIT) test, which can be used to analyse mitochondrial
activity in living cells.
To test whether the NMDAreceptor was
involved in the damage done by ASM, the receptor was blocked by
(±)2-amino-5 phosphonopentanocid (AP5).
Our results showed damage/cell death
from an added quantity of 0,06 mg/ml ASM each day for 4 days.
As a comparison there is 0,24 mg/rnl ASM
in Cola light MTT- and LDH-tests showed damage to the neurons at an
added quantity of 1.5 and 3.00 mg/ml ASM after 22 hours of
incubation. The results also show that ASM is in part acting through
the NMDA-receptor because AP5 reduced or blocked the damage to the
Conclusion: In light of these results our conclusion is that
in order to be on the safe side it should be warned against use
of ASM as a food additive, maybe especially in products consumed
by children, because NMDA-receptors and the synapses involved also
are connected to learning.
Elisabeth Hetle og Arnstein Eltervaag HOVedonneave 2001