by Dr. Joseph Mercola
January 17, 2022
SARS-CoV-2 has been isolated, photographed, genetically sequenced,
and exists as a pathogenic entity
The U.S. Centers for Disease Control and Prevention grows the virus
in cell culture to ensure widespread availability for researchers
who want to study it
At least part of the confusion appears to be rooted in how the term
"isolated" is defined. Some insist a virus is not isolated unless
it's also purified, while others say a virus doesn't have to be
purified in order to be "isolated"
Another sticking point for some is whether or not SARS-CoV-2 has
ever been isolated from a human subject without passing it through
animal cells, as such media could be contaminated and therefore the
source of the virus
Researchers have verified that the genetic sequence of the virus
obtained from the American Type Culture Collection, a global
resource center for reference microorganisms, is an exact match to
the virus found in people with symptomatic COVID-19
While some still claim
SARS-CoV-2 doesn't actually exist, this seems
to fly in the face of several well-established facts.
The virus has
actually been photomicrographed, 1,2 whole-genome sequences of the
various strains are available, 3,4 and with the appropriate
credentials anyone can obtain the live virus to conduct research.
While I am absolutely no fan of the U.S. Centers for Disease
Control and Prevention (CDC),
they do grow the virus in cell culture to ensure widespread
availability for researchers who want to study it. 5
Examples of research where you need the actual virus include,
virus stability research
What's the Confusion?
At least part of the confusion appears to be rooted in how the term
"isolated" is defined.
Some insist a virus is not isolated unless
it's also purified, while others say a virus doesn't have to be
purified in order to be "isolated."
Steve Kirsch claims to have asked several experts about this, noting
that all, including Dr. Robert Malone and Dr. Li-Meng Yan, say that
the virus has indeed been "isolated."
"So, it has been 'isolated'
according to their belief in what the term means," Kirsch writes.
"Others interpret the term differently and would claim the virus
hasn't been isolated.
In fact, according to their definition,
virus in history has ever been isolated...
That's important to know!
They use that as justification for their belief that there is no
virus here since viruses don't exist at all."
When Kirsch asked his readers for input, one pointed out: 8
"The real question
has it been
isolated from a HUMAN subject w/o passing it through (say)
Monkey Kidney Cells?
Because there is
plenty of evidence out there that says it hasn't been isolated
directly (no intermediaries) from a HUMAN subject."
According to Kirsch, the scientists he spoke with did not agree that
this was a concern, and,
"Sabine Hazan verified that the sequence of
the virus obtained from ATCC [the American Type Culture Collection,
a global resource center for reference microorganisms] matched
exactly what she found in people who have the virus." 9
As noted in Sabine Hazan's paper, "Detection of SARS-CoV-2 From Patient
Fecal Samples by Whole Genome Sequencing": 10
"Study participants underwent testing for SARS-CoV-2 from fecal
samples by whole genome enrichment NGS [next-generation sequencing]
(n = 14), and RT-PCR nasopharyngeal swab analysis (n = 12).
The concordance of SARS-CoV-2 detection by enrichment NGS from
stools with RT-PCR nasopharyngeal analysis was 100%.
were identified in four patients, with a total of 33 different
mutations among those in which SARS-CoV-2 was detected by whole
genome enrichment NGS."
Germ Theory and Terrain Theory Both Have Merit
As noted by independent journalist and political analyst Jeremy
Hammond in a March 2021 interview, 11 the claim that SARS-CoV-2 has
never been isolated and actually doesn't exist at all is perhaps one
of the most counterproductive arguments of the health freedom
By insisting that there is no virus, and that COVID-19 is caused by
things like 5G radiation alone, allows the mainstream media to
dismiss entirely legitimate concerns about
exposure (EMF) and
5G - including the possibility that it might make
some people more vulnerable to infections.
Like Hammond, I believe the pathogenesis of COVID-19 involves both
germ theory and terrain theory, not just one or the other.
"SARS-CoV-2 infection is an insufficient but necessary factor in the
pathogenesis of COVID-19," Hammond says.
"The virus is
constantly being isolated and whole genome sequenced by scientists
all over the world." 12
should be a wake-up call to the human population,
and especially the populations of developed countries,
need to focus on natural means
of maintaining good health and
in greater harmony with
our natural environment.
That said, environmental factors can clearly play a role, in that
they can make you more or less predisposed to severe infection when
you encounter this virus.
...and much more.
Hammond argues that the,
should be a wake-up call to the human population, and especially
the populations of developed countries, about the need to focus
on natural means of maintaining good health and living in
greater harmony with our natural environment."
And, as Hammond points out, pathogenic challenge is
absolutely necessary for general good health and strong immunity.
When we shield ourselves too much from everyday pathogens, we make
ourselves vulnerable to chronic diseases instead.
SARS-CoV-2 Genome Sequencing From Italy
As for whether SARS-CoV-2 has been isolated and exists as a viral
entity, the answer appears to be yes.
For example, an Italian
paper 13 published in the Journal of Virology, dated May 18, 2020,
detailed the isolation and full-length genome of the virus taken
from COVID-19 patients in Italy:
"At the beginning of
March 2020, the first nasopharyngeal swabs positive for
SARS-CoV-2 started to be detected in the Northern Eastern Region
of Friuli-Venezia Giulia...
Swab contents were
seeded on Vero E6 cells and monitored for cytopathic effect and by
an RT-PCR protocol using primers for the N region.
Cell culture supernatants from passage 1 (P1) of four isolates were
collected, and RNA was extracted with QIAamp viral RNA minikit (Qiagen)
and quantified with an in vitro-transcribed RNA standard... The
quantity and quality of the RNA were assessed...
For each sample,
100 ng of total RNA was processed using Zymo-Seq RiboFree ribosomal
depletion library preparation kit (Zymo Research).
All the obtained libraries passed quality check and were quantified
before being pooled at equimolar concentration and sequenced...
Sequenced reads that passed the quality check (Phred score ≥30) were
adaptor and quality trimmed, and the remaining reads were assembled
de novo using Megahit (v.1.2.9) with default parameter settings.
Megahit generated in all cases 7 contigs with more than 1,000 bp and
100× coverage; all of these assembled contigs were compared (using
BLASTn) against the entire nonredundant (nr) nucleotide and protein
In all cases the longest and more covered contigs were identified as
MT019532.1,14 'Severe acute respiratory syndrome coronavirus 2
isolate BetaCoV/Wuhan/IPBCAMS-WH-04/2019, complete genome,' with 99%
identity and 0 gaps.
The longer sequences were named hCoV-19/Italy/FVG/ICGEB_S1, _S5,
_S8, and _S9 and were deposited in
showed an uneven coverage along the SARS-CoV-2 genome, with an
average range from 126 to 7,576 reads and a mean coverage per
sample of 1,169×... Phylogenetic trees were inferred using the maximum
likelihood method ...
The first sequences deposited in GISAID (EPI_ISL_410545 and
EPI_ISL_410546) were collected in Rome from a Chinese tourist from
Hubei province who got infected before visiting Italy, and another
one (EPI_ISL_412974) was from a test-positive Italian citizen
returning from China.
Only two sequences were reported from
the Lombardy cluster (EPI_ISL_412973 and EPI_ISL_413489). In
this report four additional sequences from cases
epidemiologically linked to northern Italy have been examined...
Sequence analysis showed a good coverage along the
SARS-CoV-2 genome for all four isolates.
Based on the marker variant S D614G, all four sequences grouped in
the Bavarian rooted subclade G, which is dominant in Europe,
including the sequence from Lombardy, but distinct from the three
sequences mentioned above originating directly from China.
Intriguingly, the new isolates were more closely related to
EPI_ISL_412973, while EPI_ISL_413489 was more distant.
could be found for the putative 382-nucleotide (nt) deletion in ORF8
detected in Singapore, which has been proposed to indicate an
SARS-CoV-2 Genome Sequencing from Germany
Similarly, the complete genome sequence of the virus taken from a
German woman has been published, this one in the journal
Microbiology Resource Announcements, in June 2020.
Here, an oropharyngeal swab sample from a female patient who tested
positive but had no symptoms at the time of the test was used to
isolate the strain. 15
Table 1 in the paper compares the nucleotide
variants found in the sampled virus and those of a reference strain
already logged in the gene bank.
Another paper 16 in Annals of Internal Medicine, published in August
2020, isolated the virus from ocular (eye) secretions of an Italian COVID
"The patient, a 65-year-old woman, travelled from Wuhan, China, to
Italy on 23 January 2020 and was admitted on 29 January 2020, 1 day
after symptom onset.
At admission to the high isolation unit... she
presented with nonproductive cough, sore throat, coryza, and
She had no fever until day 4, when fever
(38°C), nausea, and vomiting began.
Infection with SARS-CoV-2 was confirmed by performing real-time
reverse transcription polymerase chain reaction (RT-PCR) assay on
sputum samples (cycle threshold value [Ct], 16.1) on the admission
day, followed by viral M gene sequencing (GenBank accession number
MT008022), and virus isolation on Vero E6 cell line
The full genome sequence was obtained from either clinical sample or
culture isolate (GISAID accession numbers EPI_ISL_410545 and
Genome Sequencing from India and Colombia
SARS-CoV-2 has also been isolated from the urine of a COVID-19
A November 2020 paper 19 sought to determine,
various clinical specimens obtained from COVID-19 patients contain
the infectious virus," and found SARS-CoV-2 RNA "in all naso/oropharyngeal
swabs and saliva, urine and stool samples collected between Days
8 and 30 of the clinical course."
Viable SARS-CoV-2 was also found in the nasal washes of ferrets that
had been inoculated with urine or stool from a COVID-19 patient.
virus has also been isolated by researchers in the U.S., 20 China,
India, 22 Canada, 23 Australia, 24 Korea
25 and Colombia. 26
The Colombian paper reads in part: 27
"Objective: To describe the isolation and characterization of an
early SARS-CoV-2 isolate from the epidemic in Colombia.
and methods: A nasopharyngeal specimen from a COVID-19 positive
patient was inoculated on different cell lines.
To confirm the presence of SARS-CoV-2 on cultures we used qRT-PCR,
indirect immunofluorescence assay, transmission and scanning
electron microscopy, and next-generation sequencing.
Results: We determined the isolation of SARS-CoV-2 in Vero-E6 cells
by the appearance of the cytopathic effect three days post-infection
and confirmed it by the positive results in the qRT-PCR and the
immunofluorescence with convalescent serum.
Transmission and scanning electron microscopy images obtained from
infected cells showed the presence of structures compatible with
Finally, a complete genome sequence obtained by
next-generation sequencing allowed classifying the isolate as B.1.5
The evidence presented in this article confirms the
first isolation of SARSCoV-2 in Colombia.
In addition, it shows
that this strain behaves in cell culture in a similar way to
that reported in the literature for other isolates and that its
genetic composition is consistent with the predominant variant
in the world."
If Virus Exists, Why aren't Certain Studies Done?
As mentioned earlier, the actual virus is needed in order to conduct
Now, since the virus does exist, we also ought to
be able to conduct studies to assess whether the COVID shots cause
antibody dependent enhancement (ADE).
As suggested by Kirsch, 28
"Give the vaccine to the animals, wait,
then expose them to the virus" and see what happens.
Does it prevent
infection and transmission, or does it make the animals more prone
If the animals got sicker, that would be evidence of
ADE, a problem that has plagued coronavirus vaccine research for
It's why we don't have a vaccine against the common cold, caused by coronaviruses... Remarkably, this animal research has never been done
for the COVID shots.
The question is, why...?
Kirsch believes the answer
"nobody wants to know
the answer... The top management of the FDA knows it would kill
the vaccine program if they did this."
On the other hand, the vaccinated, just like the unvaccinated, tend
to experience only mild symptoms with Omicron...
So, perhaps the shots
aren't causing ADE (which could turn even a milder variant into
However, ADE is far from the only concern.
Clearly, these shots are
associated with a dramatically increased risk of cardiovascular,
cardiac and neurological problems.
These too could be confirmed
through animal studies - rather than testing on our children - and
we wouldn't even need the virus for those.
Either way, I believe it's scientifically accurate to claim that,
SARS-CoV-2 has been isolated, genetically sequenced, and that it
exists as a pathogenic entity...
Getting too far into the weeds of
theories that refute the existence of viruses altogether, will only
slow down and hamper the truth movement rather than aid it along,
and I would strongly discourage anyone from engaging in this highly
Additional Third Party Information
January 19, 2021
Information sent by
Virus-Isolation Is It Real?
Andrew Kaufman MD responds to Jeremy Hammond
Sources and References
1, 7, 8, 28
Kirsch Substack January 9, 2022
January 24, 2020
Bank SARS-CoV-2 isolate, complete genome
5, 6 CDC
9, 10 Gut
Pathogens 2021; 13(7)
11, 12 Jeremy
Hammond March 9, 2021
of Virology May 18, 2020 DOI: 10.1128/JVI.00543-20
Resource Announcements June 2020; 9(23): e00520-20
16, 17 Annals
of Internal Medicine August 4, 2020 DOI: 10.7326/M20-1176
Microbes & Infections December 2020; 9(1):991-993
Microbiology & Infections November 2020;26(11):1520-1524
March 7, 2020 DOI: 10.1101/2020.03.02.972935
Weekly February 15, 2020 DOI: 10.46234/ccdcw2020.033
Journal of Medical Research February & March 2020; 151(2 &
Infectious Diseases September 2020; 26(9): 2054–2063
Medical Journal of Australia July 28, 2021
Public Health Research Perspectives February 2020; 11(1):
26, 27 Biomedica
October 30, 2020; 40(Supl. 2):148-158